Enzymatic hydrolysis for the removal of 3- monochloropropanediol esters in edible oils using Candida rugosa lipase in the presence of deep eutectic solvents and nanocellulose

3-monochloropropanediol ester (3-MCPDE) is identified as a food-borne contaminant in edible oils and are classified possible carcinogen. This study reports an efficient enzymatic technique for the removal of 3-MCPDE from extra virgin olive oil (EVO) using Candida rugosa lipase (CRL) biocatalyst presence choline chloride fructose-based natural deep eutectic solvent (NADES) nanocellulose (NC) extracted almond shells. The validity method was confirmed by gas chromatography mass spectrometry (GC-MS) showing adequate precision with relative standard deviation values ≤ 2.37%. quantification detection limit within permissible levels 3-MCPD oils. Under optimized conditions 30 min at 90 °C 60 µL phenylboronic acid (PBA), hydrolysis resulted 79.8% spiked EVO. No adverse effects EVO were detected this respect to quality testing. application DESs NCs support material CRL has yet be explored. could have significant impact on industry producing higher free 3-MCPDE.